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CC 49 [CC-49, CC49]
CC 49 [CC-49, CC49]
規格:
價格:
編號:B164125
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CC 49 [CC-49, CC49]
商品貨號
B164125
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format
frozen
培養基
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188).
Spleen cells were fused with NS-1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein
Comments
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188).
Spleen cells were fused with NS-1 mouse myeloma cells.
The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
mouse IgG1 kappa
Name of Depositor
The United States of America
U.S. Patent Number
References

Schlom J, Colcher D. Second generation monoclonal antibodies having binding specificity to TAG-72 and human carcinomas and methods for employing the same. US Patent 5,512,443 dated Apr 30 1996

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