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CBL-1
CBL-1
規格:
價格:
編號:B164124
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CBL-1
商品貨號
B164124
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody binds to and blocks the action of the growth factor.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a 15000 dalton human autocrine growth factor of tumor cells and activated lymphocytes
Cellular Products
immunoglobulin; monoclonal antibody; against a 15000 dalton human autocrine growth factor of tumor cells and activated lymphocytes
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with human acute leukemia cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody binds to and blocks the action of the growth factor.
Complete Growth Medium
CULTURE MEDIUM: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgM
Name of Depositor
R Billing, PI Terasaki, Regents of the University of California
U.S. Patent Number
References

Billing RJ. Monoclonal antibodies to an autocrine growth factor antigen that binds to activated lymphocytes and cancer cells. US Patent 5,330,896 dated Jul 19 1994

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