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CATH.a

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價格:

編號:B164123

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

CATH.a

商品貨號

B164123

Organism

Mus musculus, mouse

Tissue

brain

培養基

Cell Type

neuron

Product Format

frozen

Culture Properties

mixed: adherent and suspension

Biosafety Level

2 [Cells contain SV-40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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Derivation

The CATH.a cell line was established from cultures of a tumor that arose in the brain of a transgenic mouse.

Genes Expressed

dopamine; norepinephrine; synaptophysin

Tumorigenic

Yes

Effects

Yes, in syngeneic mice

Comments

The transgene contain the SV40 T antigen coding sequence linked to a 783 bp portion of the rat tyrosine hydroxylase gene.

The cells express tyrosine hydroxylase and dopamine beta-hydroxylase, and contain neurofilaments.

Complete Growth Medium

The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001). To make the complete medium add the following components to the base medium (88% final concentration):

Horse Serum (ATCC 30-2040), for an 8% final concentration
Fetal Bovine Serum (FBS; ATCC 30-2020), for a 4% final concentration

Subculturing

Cultures are a mixture of adherent and suspended cells. Collect the floating cells and transfer to new flasks. Dissociate the attached cells using 0.12% trypsin without EDTA. Remove Trypsin by neutralization and gentle centrifugation followed by resuspension in complete growth medium and add to the suspension cell population.

Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Cryopreservation

Freeze medium: 85% RPMI-1640 (ATCC 30-2001) + 10% FBS (ATCC 30-2020) + 5% DMSO (ATCC 4-X)

Storage Temperature: Liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Name of Depositor

Trustees of Tufts University

Deposited As

Mus musculus

U.S. Patent Number

5,591,626

References

Chikaraishi DM. Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene. US Patent 5,591,626 dated Jan 7 1997

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