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Caki-2

規(guī)格:

價格:

編號:B164111

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Caki-2

商品貨號

B164111

Organism

Homo sapiens, human

Tissue

kidney

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

clear cell carcinoma

Age

69 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

(P8) hypopentaploid to hypohexaploid (+A2, +A3, +B, +C, +D, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, minutes, breaks, and large subtelocentric markers

Images

Derivation

According to the originator, Caki-2 was established from a primary clear cell carcinoma of the kidney.

This is another line isolated from a primary renal carcinoma by J. Fogh as indicated in the description for ATCC HTB-46.

Clinical Data

69 years

Caucasian

male

Antigen Expression

Antigen expression: Blood Type A; Rh-

Tumorigenic

Yes

Effects

Yes, in nude mice; forms clear cell carcinoma

Comments

Recent evaluation (K. Pulkkanen and J. Parkinen, personal communication) of nude mouse tumors formed by this line in orthotopic and s.c. implantations were consistent with cystic papillary renal cell carcinoma according to the criteria of Kovacs et al.

Ultrastructural features reported include microvilli and microfilaments with few mitochondria, lysosomes or lipid droplets.

Frequent multilamellar bodies were observed, but no virus particles were seen.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Culture medium, 95%; DMSO, 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10

D16S539: 9,13

D5S818: 11

D7S820: 12

THO1: 6

TPOX: 9,11

vWA: 16,17

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Name of Depositor

J Fogh

Deposited As

Homo sapiens

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Kovacs G, et al. The Heidelberg classification of renal cell tumors. J. Pathol. 183: 131-133, 1997. PubMed: 9390023

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