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當(dāng)前位置: 首頁 > ATCC代理 > CAB 117-12D10 [12D10] B164107
CAB 117-12D10 [12D10]
CAB 117-12D10 [12D10]
規(guī)格:
價(jià)格:
編號:B164107
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CAB 117-12D10 [12D10]
商品貨號
B164107
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma:lymphoblast B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
adult
Gender
female
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen.
It is specific to amino acids 195-208 on the factor VII/VIIa molecule.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Clinical Data
female
Genes Expressed
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa)
Cellular Products
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa)
Comments
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen.
It is specific to amino acids 195-208 on the factor VII/VIIa molecule.
Complete Growth Medium
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 kappa
Name of Depositor
Corvas, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Soule HR, Brunck TK. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex. US Patent 5,506,134 dated Apr 9 1996

Biemond BJ, et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb. Haemostasis 73: 223-230, 1995. PubMed: 7792734

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