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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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C58(NT)D.1.G.OUA<sup>R</sup>.1
C58(NT)D.1.G.OUA<sup>R</sup>.1
規格:
價格:
編號:B164096
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C58(NT)D.1.G.OUAR.1
商品貨號
B164096
Organism
Rattus norvegicus, rat
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
WFu
Applications
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Storage Conditions
liquid nitrogen vapor temperature
Comments
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
R Hyman
Deposited As
Rattus sp.
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480

Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188

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