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C5/MJ
C5/MJ
規格:
價格:
編號:B164095
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C5/MJ
商品貨號
B164095
Organism
Homo sapiens, human
Tissue
cord blood
培養基
Cell Type
T lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain Retrovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Age
newborn
Gender
male
Applications
The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was established by Popovic et al. in 1983 from neonatal cord blood by co-cultivation with an HTLV-I producing cell line, MJ (see ATCC CRL-8294).
Clinical Data
male
neonatal
Comments

The cells express a cell surface glycoprotein of 61000 daltons (45000 daltons when not glycosylated) that is characteristic of HTLV infection.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at about 2 to 4 x 105 cells/mL and maintain between 4 x 105 and 1 x 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 12
D16S539: 8,11
D5S818: 13
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 16,17
Name of Depositor
President and Fellows of Harvard College
Deposited As
Homo sapiens
U.S. Patent Number
Year of Origin
1983
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

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