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C-4 I
C-4 I
規(guī)格:
價格:
編號:B164092
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
C-4 I
商品貨號
B164092
Organism
Homo sapiens, human
Tissue
cervix
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain human papilloma virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma
Age
41 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
This is one of two lines developed from this patient (see ATCC CRL 1595).
Clinical Data
This is one of two lines developed from this patient (see ATCC CRL-1595).
female
Caucasian
41 years
Tumorigenic
Yes
Effects
Yes, forms tumors in nude mice
Comments
This is one of two lines developed from this patient (see ATCC CRL-1595).

The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA. ATCC confirmed this cell line is positive for the presence of HPV viral DNA sequences via PCR.


Complete Growth Medium
Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 9,11
D7S820: 10,11
THO1: 9.3
TPOX: 10,11
vWA: 14
Isoenzymes
G6PD, B
Name of Depositor
N Auersperg
Deposited As
Homo sapiens
References

Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Herz F, et al. Chromosome analysis and alkaline phosphatase of C41, a cell line of human cervical origin distinct from HeLa. Cancer Res. 37: 3209-3213, 1977. PubMed: 560251

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380

Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382

Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250

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