国产精品第七页,免费av在线播放,久久人爽

C-33A [c-33a]

規格:

價格:

編號:B164085

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

C-33A [c-33a]

商品貨號

B164085

Organism

Homo sapiens, human

Tissue

cervix

培養基

Cell Type

Epithelial, Retinoblastoma

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

carcinoma

Age

66 years adult

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 70% of cells examined. Polyploid cells occurred at 8.6%. Seven marker chromosomes were consistently detected per pseudodiploid cell. They are: t(1q17q), t(1p21q), del(18) (q21.3), der(1)t(1;17) (p16;q21.3) and three others. Several other markers were also found but they occurred only once in 15 metaphases analyzed. Neither DMs nor HSRs were detected. Structurally normal N1 was absent. Generally there are two X chromosomes in each cell.

Clinical Data

66 years adult

Caucasian

female

Oncogene

p53 +; pRB +

Genes Expressed

Oncogenes: p53 +; pRB +

Tumorigenic

Yes

Effects

Yes, in nude mice; forms undifferentiated carcinoma

Comments

The line exhibited a hypodiploid karyotype initially, and an epithelial morphology.

Karyological instability was observed with continued passage.

The retinoblastoma protein (pRB) is present but abnormal in size.

Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution.

The cells are negative for human papillomavirus DNA and RNA.

The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 13

D16S539: 13,14

D5S818: 11,12

D7S820: 10

THO1: 7,8

TPOX: 9

vWA: 18,20

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1

Name of Depositor

N Auersperg

Deposited As

Homo sapiens

Passage History

The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies. The line exhibited a hypodiploid karyotype initially, and an epithelial morphology. Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size. Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution. The cells are negative for human papillomavirus DNA and RNA.

References

J. Natl. Cancer Inst. 32: 135-148, 1964.

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479

<dfn id="0w3vl"></dfn>