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C273
C273
規(guī)格:
價(jià)格:
編號:B164079
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
C273
商品貨號
B164079
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
C273 is was derived from the Sp2/0 hybridoma cell line.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
C273 is was derived from the Sp2/0 hybridoma cell line.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin))
Cellular Products
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin))
Comments
C273 is was derived from the Sp2/0 hybridoma cell line.
It is transfected with plasmids pING2101 and pING2106.
The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Xoma Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Robinson RR, et al. Chimeric antibody with specificity to human B cell surface antigen. US Patent 5,500,362 dated Mar 19 1996

Clark EA, et al. Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456

Profous-Juchelka H, et al. Recombinant and native group B eimeria tenella immunogens useful as coccidiosis vaccines. US Patent 5,824,656 dated Oct 20 1998

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