Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Useful for visualization of sarcoma virus-induced foci and for an in vitro quantitative assay for bovine papilloma virus.
Host for transfection with plasmids containing bovine papilloma virus DNA.
C127I cells are suitable hosts for transformation with bovine papilloma virus DNA plasmids (ATCC 37110, ATCC 37111, ATCC 37112, ATCC 39475)
Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; fetal bovine serum, 10%
Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref
Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of DMEM, is ATCC catalog No. 30-2601.
Sarver N, et al. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol. Cell. Biol. 1: 486-496, 1981. PubMed: 6100967
Lowy DR, et al. Helper-independent tranformation by unintegrated Harvey sarcoma virus DNA. J. Virol. 26: 291-298, 1978. PubMed: 26810
Dvoretzky I, et al. A quantitative in Vitro focus assay for bovine papilloma virus. Virology 103: 369-375, 1980. PubMed: 6247821
Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
