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BT-483
BT-483
規(guī)格:
價(jià)格:
編號(hào):B164051
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
BT-483
商品貨號(hào)
B164051
Organism
Homo sapiens, human
Tissue
mammary gland; breast
培養(yǎng)基
Cell Type
Epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
ductal carcinoma
Age
23 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 72; range = 46 to 130
Images
Derivation
The BT-483 line was isolated by E.Y. Lasfargues and W.G. Coutinho from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer.
Clinical Data
23 years
Caucasian
female
Tumorigenic
Yes
Effects
Yes, in Amsterdam/IMR rats
Yes, in nude mice
Yes, in soft agar
Comments
The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37°C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 2
PGM3, 2
Name of Depositor
EY Lasfargues
Deposited As
Homo sapiens
References

Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

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