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BL-3
BL-3
規(guī)格:
價格:
編號:B164035
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
BL-3
商品貨號
B164035
Organism
Bos taurus, cow
Cell Type
B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells contain Bovine Viral Diarrhea Virus (BVDV)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphosarcoma; leukemia
Age
3 months
Gender
male
Strain
Hereford
Applications
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Comments
The cells do not produce bovine leukemia virus.
They are of possible use in making vaccines against bovine leukemia virus.
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
Regents of the University of California
Deposited As
Bos taurus
U.S. Patent Number
References

Theilen GH, et al. Bovine leukemia: establishment and morphologic characterization of continuous cell suspension culture, BL-1. J. Natl. Cancer Inst. 40: 737-749, 1968. PubMed: 4171552

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

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