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BF-2
BF-2
規格:
價格:
編號:B164031
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BF-2
商品貨號
B164031
Organism
Lepomis macrochirus, bluegill
Tissue
caudal trunk
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
1 year
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Virus Resistance
poliovirus 1
Complete Growth Medium
Minimum essential medium (Eagle) in Earle's BSS, 90%; fetal bovine serum; 10%
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Transfer floating cells to new flasks.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. 
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 23°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: At the time of subcultivation

Culture Conditions
Temperature: 23°C
Name of Depositor
K Wolf
Deposited As
Lepomis macrochirus
References

Wolf K, et al. Lymphocystis virus: isolation and propagation in centrarchid fish cell lines. Science 151: 1004-1005, 1966. PubMed: 4955835

Zwillenberg LO, Wolf K. Ultrastructure of lymphocystis virus. J. Virol. 2: 393-399, 1968. PubMed: 4986903

Wolf K, et al. Tadpole edema virus: a viscerotropic pathogen for anuran amphibians. J. Infect. Dis. 118: 253-262, 1968. PubMed: 5674065

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