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BF-11
BF-11
規格:
價格:
編號:B164030
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BF-11
商品貨號
B164030
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds alpha, beta and asialo forms of human erythropoietin.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human erythropoietin
Cellular Products
immunoglobulin; monoclonal antibody; against human erythropoietin
Comments
Animals were immunized with human urinary erythropoietin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds alpha, beta and asialo forms of human erythropoietin.
The antibody has been given the name Epoclonalan.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
University Patents, Inc.
U.S. Patent Number
References

Weiss TL, et al. Characterization of a monoclonal antibody to human erythropoietin. Proc. Natl. Acad. Sci. USA 79: 5465-5469, 1982. PubMed: 6957875

Goldwasser E, et al. Monoclonal anti-erythropoietin. US Patent 4,558,005 dated Dec 10 1985

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