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Bet-2
Bet-2
規格:
價格:
編號:B164027
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Bet-2
商品貨號
B164027
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? Fischer (B cell); BALB/c (myeloma)

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse IgM
Cellular Products
immunoglobulin; monoclonal antibody; against mouse IgM
Comments
Animals were immunized with TEPC 183 myeloma protein.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
JT Kung
Deposited As
rat (B cell); mouse (myeloma)
References

Kung JT, et al. A mouse IgM allotypic determinant (Igh-6.5) recognized by a monoclonal rat antibody. J. Immunol. 127: 873-876, 1981. PubMed: 6167630

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