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bcd mab23
bcd mab23
規(guī)格:
價格:
編號:B164014
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
bcd mab23
商品貨號
B164014
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Derivation
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Cellular Products
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster
Comments
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
W Driever
Deposited As
mouse (B cell); mouse (myeloma)
References

Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244

Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245

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