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BB
BB
規格:
價格:
編號:B163998
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BB
商品貨號
B163998
Organism
Ictalurus nebulosus, bullhead, brown
Product Format
frozen
培養基
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
2 years
Virus Resistance
herpes simplex; poliovirus 2; vaccinia
Comments
BB cells will grow at 4 to 34C with an optimum of between 25 and 30C.
The cells do not do well when cultured on a glass surface.
Complete Growth Medium
Minimum essential medium (Eagle) with 2mM L-glutamine and Hanks' BSS with 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 23.0°C
Name of Depositor
RG Malsberger
Deposited As
Ictalurus nebulosus
References

Wolf K, Quimby MC. Fish cell and tissue culture In: Wolf K, Quimby MC. Fish physiology, 3 New York Academic Press pp. 253-301, 1969

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