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BA-G5
BA-G5
規格:
價格:
編號:B163991
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BA-G5
商品貨號
B163991
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with rat cardiac muscle alpha myosin heavy chain but not with rat skeletal muscle myosins.
Storage Conditions
liquid nitrogen vapor temperature
Derivation
Spleen cells were fused with NSO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat cardiac alpha myosin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against rat cardiac alpha myosin heavy chain
Comments
Animals were immunized with bovine fetal skeletal muscle myosin.
Spleen cells were fused with NSO myeloma cells.
The antibody reacts with rat cardiac muscle alpha myosin heavy chain but not with rat skeletal muscle myosins.
The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b , IgG2b
Name of Depositor
S Schiaffino
Deposited As
mouse (B cell); mouse (myeloma)
References

Rudnicki MA, et al. Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells. Dev. Biol. 138: 348-358, 1990. PubMed: 2318340

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