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BA7.3C.9
BA7.3C.9
規格:
價格:
編號:B163990
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BA7.3C.9
商品貨號
B163990
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Erythroleukemia
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody is specific for human stem cell factor (hSCF) receptor.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human stem cell factor (hSCF) receptor (CD117)
Cellular Products
immunoglobulin; monoclonal antibody; against human stem cell factor (hSCF) receptor (CD117)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with the erythroleukemia cell line OCIM1 because of their high SCF receptor display.
Spleen cells were fused with NS-1 mouse myeloma cells.
The antibody is specific for human stem cell factor (hSCF) receptor.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
Amgen, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Broudy VC, Lin N. Method of reconstituting hematopoietic cells using monoclonal antibodies to the stem cell factor receptor. US Patent 5,906,938 dated May 25 1999

Broudy VC, Lin N. Monoclonal antibodies to stem cell factor receptors. US Patent 5,919,911 dated Jul 6 1999

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