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B72.3
B72.3
規格:
價格:
編號:B163986
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B72.3
商品貨號
B163986
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Applications
The antibody reacts with extracts of Met 1 but not Met 2 or with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells or with liver extracts or a variety of live normal and sarcoma cells.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human mammary tumor cells
Cellular Products
immunoglobulin; monoclonal antibody; against human mammary tumor cells
Comments
Animals were immunized with membrane enriched fractions of extracts from either of two human breast tumor metastases to the liver (Met 1 and Met 2).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with extracts of Met 1 but not Met 2 or with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells or with liver extracts or a variety of live normal and sarcoma cells.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 95; DMSO, 5%
Isotype
IgG1
Name of Depositor
NIH
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Colcher D, et al. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199-3203, 1981. PubMed: 6789331

Colcher D, et al. Process for producing monoclonal antibodies reactive with human breast cancer. US Patent 4,522,918 dated Jun 11 1985

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