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B69
B69
規格:
價格:
編號:B163985
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B69
商品貨號
B163985
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to and neutralizes most strains of IBD virus.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Infectious Bursal Disease (IBD) virus
Cellular Products
immunoglobulin; monoclonal antibody; against Infectious Bursal Disease (IBD) virus
Comments
Animals were immunized with IBD virus.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to and neutralizes most strains of IBD virus.
It does not react with a newly identified variant of IBD virus.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15% - OR - RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a; kappa light chain
Name of Depositor
Univ. of Maryland
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Snyder DB. Novel infectious bursal disease virus. US Patent 5,064,646 dated Nov 12 1991

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