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當(dāng)前位置: 首頁 > ATCC代理 > B27M1 B163978
B27M1
B27M1
規(guī)格:
價(jià)格:
編號(hào):B163978
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
B27M1
商品貨號(hào)
B163978
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/08A2 myeloma cells.
Clinical Data
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic
Comments
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Spleen cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for HLA B27 and for HLA B7 at appropriate dilution.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
F Grumet
Deposited As
mouse (B cell); mouse (myeloma)
References

Grumet FC, et al. Monoclonal anti-HLA-B27 antibody (B27M1): production and lack of detectable typing difference between patients with ankylosing spondylitis, Reiter's syndrome, and normal controls. Lancet 2: 174-176, 1981. PubMed: 6166821

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