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AtT-20

規(guī)格:

價(jià)格:

編號(hào):B163962

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

AtT-20

商品貨號(hào)

B163962

Organism

Mus musculus, mouse

Tissue

pituitary

培養(yǎng)基

Product Format

frozen

Morphology

small rounded cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

tumor

Strain

LAF1

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

Clone AtT-20, an ACTH secreting cell line, was cloned in October, 1966, by G. Sato and associates from earlier cultures established after alternate passage of mouse pituitary tumor cells as tumors in animals and in cell culture. (The cells were cultured in Ham's F10 medium, 82.5%; horse serum, 15%; FBS, 2.5%). The mouse pituitary tumor was originally established in LAF1 mice by J. Furth, et al. This clone has now been carried in culture without alternate animal passage.

Genes Expressed

adrenocorticotropic hormone (ACTH)

Cellular Products

adrenocorticotropic hormone (ACTH)

Virus Susceptibility

Vesicular stomatitis virus
Human poliovirus 1

Comments

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Subculturing

Allow clusters to settle and remove all but 5 to 10 mL of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Note: The cells do not attach but aggregate to form clusters; the clusters are viable.

Cryopreservation

Freeze medium: culture medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Name of Depositor

G Sato

Deposited As

Mus musculus

Passage History

This clone has now been carried in culture without alternate animal passage.

Year of Origin

October, 1966

References

Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682

Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002

Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327

Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556

B163964：AtT-20ins (CGT-6)

B163963：AtT-20/D16v-F2

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