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AsPC-1
AsPC-1
規格:
價格:
編號:B163960
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AsPC-1
商品貨號
B163960
Organism
Homo sapiens, human
Tissue
pancreas; derived from metastatic site: ascites
培養基
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
62 years
Gender
female
Ethnicity
Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Clinical Data
62 years
Caucasian
female

Genes Expressed
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Cellular Products
carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,13
    D13S317: 9,12
    D16S539: 11
    D5S818: 12
    D7S820: 12,13
    THO1: 7,9.3
    TPOX: 8,10
    vWA: 17
    Name of Depositor
    MH Tan
    Deposited As
    Homo sapiens
    References

    Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348

    Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528

    Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

    Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

    Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565

    Cross References

    Nucleotide (GenBank) : AA640215 01 subtracted 3' EST library Homo sapiens cDNA clone U32, mRNA sequence.

    Nucleotide (GenBank) : AA640216 02 subtracted 3' EST library Homo sapiens cDNA clone U30, mRNA sequence.

    Nucleotide (GenBank) : AA640217 03 subtracted 3' EST library Homo sapiens cDNA clone D32, mRNA sequence.

    Nucleotide (GenBank) : AA640218 04 subtracted 3' EST library Homo sapiens cDNA clone D42, mRNA sequence.

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