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AMS 9.1.1.1
AMS 9.1.1.1
規(guī)格:
價(jià)格:
編號(hào):B163940
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
AMS 9.1.1.1
商品貨號(hào)
B163940
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with cells bearing the a, c, d,f g, h, j and n haplotypes at the Igh-C locus.
The antibody is similar in specificity to that of ATCC TIB-149 (Ig(5a)7.5).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Igh-5.4 (IgDa)
Cellular Products
immunoglobulin; monoclonal antibody; against Igh-5.4 (IgDa)
Comments
Animals were immunized with spleen cells from BALB/c mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with cells bearing the a, c, d,f g, h, j and n haplotypes at the Igh-C locus.
The antibody is similar in specificity to that of ATCC TIB-149 (Ig(5a)7.5).
Also known as 10-4-22).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Isotype
IgG2b
Name of Depositor
AM Stall
Deposited As
mouse (B cell); mouse (myeloma)
References

Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J. Immunol. 132: 787-795, 1984. PubMed: 6690619

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