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AMJ2-C8

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價格:

編號:B163937

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

AMJ2-C8

商品貨號

B163937

Organism

Mus musculus, mouse

Tissue

lung

培養基

Cell Type

macrophage (alveolar); infected with J2 virus

Product Format

frozen

Morphology

macrophage

Culture Properties

suspension; some adherent cells

Biosafety Level

2 [Cells contain J2 murine retrovirus viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

10 weeks old

Gender

female

Strain

C57BL/6J

Applications

Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.

Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.

AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.

They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.

Storage Conditions

liquid nitrogen vapor phase

Images

Clinical Data

female

Antigen Expression

MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -

Genes Expressed

MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -

Comments

AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.

Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.

Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.

AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).

AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.

The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%

Subculturing

Scrape off the attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

Name of Depositor

AV Palleroni

Deposited As

mouse

References

Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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