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AF6-78.25.4
AF6-78.25.4
規格:
價格:
編號:B163918
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AF6-78.25.4
商品貨號
B163918
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with cells bearing the b, d and n haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Igh-6.6 (IgMb)
Cellular Products
immunoglobulin; monoclonal antibody; against Igh-6.6 (IgMb)
Comments
Animals were immunized with spleen cells from C57BL/10J.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with cells bearing the b, d and n haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
AM Stall
Deposited As
mouse (B cell); mouse (myeloma)
References

Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J. Immunol. 132: 787-795, 1984. PubMed: 6690619

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