Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain
Strain:? BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody binds to residues A8-10 (THR,SER,ILE) of human insulin.
The antibody cross-reacts with insulin from pig, rabbit and rat and pro-insulin from cow and pig.
Tested and found negative for ectromelia virus (mousepox).
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human insulin (residues A8-10)
Cellular Products
immunoglobulin; monoclonal antibody; against human insulin (residues A8-10)
Comments
Animals were immunized with zinc containing crystalline human insulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to residues A8-10 (THR,SER,ILE) of human insulin.
The antibody cross-reacts with insulin from pig, rabbit and rat and pro-insulin from cow and pig.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
