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AB1-2
AB1-2
規格:
價格:
編號:B163902
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AB1-2
商品貨號
B163902
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
lymph node
培養基
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
A/J
Applications
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
Genes Expressed
immunoglobulin; monoclonal antibody; against T15 idiotype
Cellular Products
immunoglobulin; monoclonal antibody; against T15 idiotype
Comments
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
mouse IgG1 kappa , IgG1; kappa light chain
Name of Depositor
JF Kearney
Deposited As
mouse (B cell); mouse (myeloma)
References

Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186

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