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A-72
A-72
規(guī)格:
價格:
編號:B163892
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
A-72
商品貨號
B163892
Organism
Canis familiaris, dog
Tissue
unknown
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
tumor
Age
8 years
Gender
female
Strain
Golden retriever
Applications

efficacy testing Ref Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever.
Clinical Data
female
Virus Susceptibility
Canine adenovirus 2
Canine coronavirus , Canine coronavirus
Canine parainfluenza virus
Canid herpesvirus 1
Canine distemper virus
Canine minute virus
Comments
A canine parvovirus related to Feline panleukopenia virus has been recovered from A-72 cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week

Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37°C
Name of Depositor
LN Binn
Deposited As
Canis familiaris
References

Binn LN, et al. Establishment of a canine cell line: derivation, characterization, and viral spectrum. Am. J. Vet. Res. 41: 855-860, 1980. PubMed: 6254399

The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever.

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

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