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A6 [A-6]
A6 [A-6]
規(guī)格:
價格:
編號:B163887
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
A6 [A-6]
商品貨號
B163887
Organism
Homo sapiens; Mus musculus, human; mouse
Cell Type
Hybridoma
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, with some loosely adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
The cells are useful as fusion partners for human B cells in the generation of human hybridomas.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
Comments
The line was produced by fusing a G-418 resistant derivative of the U-266 human myeloma cell line with P3X63Ag8.653 mouse myeloma cells.
G-418 resistance was introduced to the human myeloma by transfection with pSV-2 neo r.
The cells are useful as fusion partners for human B cells in the generation of human hybridomas.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x10(5) viable cells/ml. Maintain cultures at a cell concentration between 1 x 10(5) and 1 x 10(6) cells/ml. Scrape attached cells if necessary.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: 75% CompleteGrowth Medium + 20% FBS + 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
H Kaplan
Deposited As
human; mouse
U.S. Patent Number
References

Kaplan HS, et al. Methods and cell lines for immortalization and monoclonal antibody production by antigen-stimulated B-lymphocytes. US Patent 4,574,116 dated Mar 4 1986

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