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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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A5.4 [1506 A5.4]
A5.4 [1506 A5.4]
規格:
價格:
編號:B163885
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
A5.4 [1506 A5.4]
商品貨號
B163885
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I.
It is useful for detecting ApoA-I in immunoblotting applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I)
Cellular Products
immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with the major protein peak (G-200 fractionation) of delipidated human high density lipoprotein (HDL).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I.
It is useful for detecting ApoA-I in immunoblotting applications.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
G Schonfeld
Deposited As
mouse (B cell); mouse (myeloma)
References

Jonas A, et al. Apolipoprotein A-I structure and lipid properties in homogeneous, reconstituted spherical and discoidal high density lipoproteins. J. Biol. Chem. 265: 22123-22129, 1990. PubMed: 2125044

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