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A3.6B10

規(guī)格:

價格:

編號:B163874

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

A3.6B10

商品貨號

B163874

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with Sp2/0-Ag14 cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152)

Cellular Products

immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152)

Comments

Animals were immunized with activated human T cell clones.

Spleen cells were fused with Sp2/0-Ag14 cells.

The antibody was able to block B7 ligand binding.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C

Isotype

IgG2a kappa , IgG2a; kappa light chain

Name of Depositor

VK Kuchroo, EA Greenfield

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

6,207,156

References

Kuchroo VK, Greenfield EA. Specific antibodies and antibody fragments. US Patent 6,207,156 dated Mar 27 2001

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