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A2E11
A2E11
規(guī)格:
價格:
編號:B163871
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
A2E11
商品貨號
B163871
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids.
Derivation
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
Genes Expressed
immunoglobulin; monoclonal antibody; against aldosterone
Cellular Products
immunoglobulin; monoclonal antibody; against aldosterone
Comments
Animals were immunized with a BSA conjugate of the 3-O carboxylmethyl oxime of aldosterone.
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol and 1 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
CE Gomez-Sanchez
Deposited As
mouse (B cell); mouse (myeloma)
References

Gomez-Sanchez CE, et al. The production of monoclonal antibodies against aldosterone. Steroids 49: 581-587, 1987. PubMed: 3453564

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