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91MS441
91MS441
規(guī)格:
價(jià)格:
編號:B163849
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
91MS441
商品貨號
B163849
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/cByJ (B cell); BALB/c (myeloma)

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an antibody to a chlamydia genus antigen (anti-idiotypic antibody)
Cellular Products
immunoglobulin; monoclonal antibody; against an antibody to a chlamydia genus antigen (anti-idiotypic antibody)
Comments
Animals were immunized with 89MS30 (GLXA-Ab1) IgG.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
University of Massachusetts
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

MacDonald B, et al. Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997

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