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89MS30
89MS30
規格:
價格:
編號:B163841
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
89MS30
商品貨號
B163841
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, with some loosely adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
trachoma
Strain

Strain: BALB/cByJ (B cell); BALB/c (myeloma)

Storage Conditions
liquid nitrogen vapor temperature
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a chlamydia genus antigen
Cellular Products
immunoglobulin; monoclonal antibody; against a chlamydia genus antigen
Comments
Animals were immunized with C. trachomatis elementary bodies.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Attached cells may be subcultured by scraping.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b lambda , IgG2b; kappa light chain
Name of Depositor
University of Massachusetts
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

MacDonald B, et al. Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997

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