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88B
88B
規(guī)格:
價(jià)格:
編號(hào):B163840
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
88B
商品貨號(hào)
B163840
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with determinants on both the gamma and delta subunits of the receptor.
And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures.
Derivation
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica
Cellular Products
immunoglobulin; monoclonal antibody; against nicotinic acetylcholine receptor of Torpedo californica
Tumorigenic
Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with purified nicotinic acetylcholine receptor of Torpedo californica.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with determinants on both the gamma and delta subunits of the receptor.
And exhibits broad species reactivity (it reacts with receptors of mouse, rat, chicken and frog), and can be used in ultrastructural and immunofluorescence localization and in immunoprecipitation and immunoblotting procedures.
The domains recognized by 88B appear to be on the cytoplasmic side of the membrane.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
SC Froehner
Deposited As
mouse (B cell); mouse (myeloma)
References

Froehner SC, et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J. Biol. Chem. 258: 7112-7120, 1983. PubMed: 6189834

Green WN, Wanamaker CP. The role of the cystine loop in acetylcholine receptor assembly. J. Biol. Chem. 272: 20945-20953, 1997. PubMed: 9252423

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