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74-22-15
74-22-15
規格:
價格:
編號:B163815
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
74-22-15
商品貨號
B163815
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts precipitates a 230000 dalton antigen from the surface of swine macrophages.
A switch variant that produces IgG2b is also available (74-22-15A, ATCC HB-142.1).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against pig macrophage and granulocyte; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against pig macrophage and granulocyte; cytotoxic
Comments
Animals were immunized with thymocytes from dd miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts precipitates a 230000 dalton antigen from the surface of swine macrophages.
A switch variant that produces IgG2b is also available (74-22-15A, ATCC HB-142.1).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
JR Lunney
Deposited As
mouse (B cell); mouse (myeloma)
References

Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988

Lunney JK, et al. Overview of the first international workshop to define swine leukocyte cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 43: 193-206, 1994. PubMed: 7856053

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