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7-34-1
7-34-1
規(guī)格:
價格:
編號:B163812
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
7-34-1
商品貨號
B163812
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic)
Comments
Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific.
It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
CM Warner
Deposited As
mouse (B cell); mouse (myeloma)
References

Lie WR, et al. Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049

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