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5E4
5E4
規格:
價格:
編號:B163795
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
5E4
商品貨號
B163795
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
VacA is a secreted protein that induces vacuolation of epithelial cells.
The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821).
The antibody can be used for ELISA and immunoprecipitation applications.
Derivation
Animals were immunized with s1-m1 purified vacuolating cytotoxin (VacA) from Helicobacter pylori strain 60190 (ATCC 49503). VacA is a secreted protein that induces vacuolation of epithelial cells.
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821). It does not recognize type s2-m2 VacA.
Genes Expressed
immunoglobulin; monoclonal antibody; against VacA
Cellular Products
immunoglobulin; monoclonal antibody; against VacA
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with s1-m1 purified vacuolating cytotoxin (VacA) from Helicobacter pylori strain 60190 (ATCC 49503). VacA is a secreted protein that induces vacuolation of epithelial cells.
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821). It does not recognize type s2-m2 VacA.
The antibody can be used for ELISA and immunoprecipitation applications.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
TL Cover
Deposited As
mouse (B cell); mouse (myeloma)
References

Vinion-Dubiel AD, et al. Antigenic diversity among Helicobacter pylori vacuolating toxins. Infect. Immun. 69: 4329-4336, 2001. PubMed: 11401970

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