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5c8
5c8
規(guī)格:
價格:
編號:B163793
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
5c8
商品貨號
B163793
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)
Cellular Products
immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)
Comments
Animals were immunized with D1.1 cells (see ATCC CRL-10915).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody recognizes an antigen present on T cells that is important to the T cell mediated activation of B cells.
The antigen is a ligand for the CD40 molecule on the B cell surface.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG2a
Name of Depositor
Trustees of Columbia University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003

. Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004

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