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520C9 [520C9.C3B10T]

規(guī)格:

價格:

編號:B163777

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

520C9 [520C9.C3B10T]

商品貨號

B163777

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Cancer,Cancer

Applications

By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms.

The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human breast cancer cells

Cellular Products

immunoglobulin; monoclonal antibody; against human breast cancer cells

Tumorigenic

Yes

Effects

Yes, forms ascites in BALB/c mice

Comments

Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

By immunofluorescence, the antibody is specific for neoplastic breast tissue with some weak cross-reaction with other neoplasms.

The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.

Complete Growth Medium

Culture Medium: Dulbecco's modified Eagle's medium (containing: 0.2 units/mL
insulin, 1 mM Na pyruvate, 0.15 mg/mL oxaloacetic acid), with 4.5 g/L glucose,
60%; NCTC 109 medium, 20%; fetal bovine serum, 20%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype

IgG1

Name of Depositor

Cetus Corp.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,753,894

References

Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988

Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002

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