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5/9 m alpha3-18

規(guī)格:

價格:

編號:B163773

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

5/9 m alpha3-18

商品貨號

B163773

Organism

Cricetulus griseus, hamster, Chinese

Tissue

ovary

培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender

female

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This line was derived from the CHO DUKX cell line.

Clinical Data

female

Genes Expressed

human colony stimulating factor (M-CSF)

Cellular Products

human colony stimulating factor (M-CSF)

Comments

The cells are resistant to methotrexate.

Complete Growth Medium

Alpha minimum essential medium with 2 mM L-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides supplemented with 200 nM MTX (Methotrexate) and 10% dialyzed fetal bovine serum (Sigma F0392)

Subculturing

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended

Medium Renewal: Two to three times weely

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Name of Depositor

Genetics Institute, Inc.

Deposited As

Cricetulus griseus

U.S. Patent Number

4,868,119

References

Clark SC, Wong GG. Hematopoietic growth factors. US Patent 4,868,119 dated Sep 19 1989

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