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4F2C13
4F2C13
規格:
價格:
編號:B163770
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4F2C13
商品貨號
B163770
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.
The antibody fixes complement and binds to Protein A.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte
Tumorigenic
Yes
Effects
Yes, in pristane primed BALB/c mice
Comments
Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.
The antibody fixes complement and binds to Protein A.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype
IgG2a; kappa light chain
Name of Depositor
A Fauci, BF Haynes
Deposited As
mouse (B cell); mouse (myeloma)
References

Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676

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