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4D11
4D11
規格:
價格:
編號:B163765
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4D11
商品貨號
B163765
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells.
The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested.
Derivation
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1)
Cellular Products
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1)
Comments
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody detects a major (approximately 50%) subset of mouse NK cells.
This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells.
The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
LH Mason
Deposited As
rat (B cell); mouse (myeloma)
References

Mason LH, et al. Natural killer (NK) cell subsets in the mouse. NK-1.1+/LGL-1+ cells restricted to lysing NK targets, whereas NK-1.1+/LGL-1- cells generate lymphokine-activated killer cells. J. Immunol. 145: 751-759, 1990. PubMed: 2365999

Mason L, et al. LGL-1: a non-polymorphic antigen expressed on a major population of mouse natural killer cells. J. Immunol. 140: 4403-4412, 1988. PubMed: 2453582

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