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46-5
46-5
規(guī)格:
價(jià)格:
編號(hào):B163755
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
46-5
商品貨號(hào)
B163755
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB, but does not react with gp120 MN.
It binds to the C5 region (positions 474 - 489) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
46-5 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120
Cellular Products
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were repeatedly immunized with recombinant gp120 (rgp120) from a single isolate of HIV-1 (IIIB).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
46-5 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB, but does not react with gp120 MN.
It binds to the C5 region (positions 474 - 489) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: To to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG1
Name of Depositor
SL Epstein
Deposited As
mouse (B cell); mouse (myeloma)
References

Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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