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455
455
規格:
價格:
編號:B163751
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
455
商品貨號
B163751
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with EGF receptors, but does not block the binding of EGF to the receptor on A-431 cells.
579, 225 or 528, see ATCC HB-8506, ATCC HB-8508 and ATCC HB-8509) to A-431 cells and binds with lower affinity than 528, 225 and 579.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Tumorigenic
Yes
Effects
Yes, in pristane primed BALB/cJ mice
Comments
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
The antibody reacts with EGF receptors, but does not block the binding of EGF to the receptor on A-431 cells.
It does not inhibit the binding of other antibodies (e.g., 579, 225 or 528, see ATCC HB-8506, ATCC HB-8508 and ATCC HB-8509) to A-431 cells and binds with lower affinity than 528, 225 and 579.
It only marginally inhibits the growth of A-431 tumor cells in vitro, and does not block EGF stimulated phosphorylation of membrane proteins.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Regents of the University of California
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990

Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961

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