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411-14E10
411-14E10
規(guī)格:
價格:
編號:B163743
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
411-14E10
商品貨號
B163743
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence SGFNADYEASSRC corresponding to peptides 4 to 17 of the fos oncogene (Note: There is a serine deletion at position 15 of the peptide antigen).
In immunoblot testing, a protein of approximately 55 kD is detected in mink cells transformed by FeSV (MSTF).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence SGFNADYEASSRC corresponding to peptides 4 to 17 of the fos oncogene (Note: There is a serine deletion at position 15 of the peptide antigen).
Spleen cells were fused with Sp2/0 myeloma cells.
In immunoblot testing, a protein of approximately 55 kD is detected in mink cells transformed by FeSV (MSTF). In HeLa cell nuclei (induced by TPA), a protein of approximately 40 kD is detected.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Dulbecco's modified Eagle's medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b; kappa light chain
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
References

Flanagan-Cato LM, McEwen BS. Pattern of Fos and Jun expression in the female rat forebrain after sexual behavior. Brain Res. 673: 53-60, 1995. PubMed: 7757479

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