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3C10
3C10
規格:
價格:
編號:B163730
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
3C10
商品貨號
B163730
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human monocytes but not with platelets, lymphocytes, dendritic cells, epithelial cells or fibroblasts.
The antibody appears to react with all tissue macrophages, and to differentiate between macrophages and dendritic cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human monocyte; against CD14; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against human monocyte; against CD14; cytotoxic
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with human peripheral blood monocytes.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antibody reacts with human monocytes but not with platelets, lymphocytes, dendritic cells, epithelial cells or fibroblasts.
The antibody appears to react with all tissue macrophages, and to differentiate between macrophages and dendritic cells.
The antibody the antibody immunoprecipitates gp55.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
RM Steinman
Deposited As
mouse (B cell); mouse (myeloma)
References

Van Voorhis WC, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J. Exp. Med. 158: 126-145, 1983. PubMed: 6190974

Van Voorhis WC, et al. Relative efficacy of human monocytes and dendritic cells as accessory cells for T cell replication. J. Exp. Med. 158: 174-191, 1983. PubMed: 6190976

Zvaifler NJ, et al. Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis. J. Clin. Invest. 76: 789-800, 1985. PubMed: 3875632

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